New Zealand Plant Protection 56 (2003): 109-112
Strains of Pseudomonas syringae able to grow on minimal medium containing 500 mg/litre of copper sulphate were selected from a collection of streptomycin-resistant strains isolated in New Zealand. From one of these copper-resistant strains a 1.3 kb fragment of DNA was isolated by PCR using primers designed on genes reported to be associated with copper resistance. Sequencing of this fragment revealed that a 572 bp and a 275 bp portion had 98% and 95% similarity with ORF A of P. syringae pv. actinidiae, a gene that codes for copper resistance. Smaller portions of this 1.3 kb fragment were highly similar (89% to 100%) to portions of another gene involved in copper resistance (copA) from Pseudomonas syringae pv. tomato. Using this 1.3 kb fragment as a probe, homologous DNA was detected in eight other strains of P. syringae, which were all subsequently found to be resistant to copper.
Keywords: Pseudomonas syringae pv actinidiae, Pseudomonas syringae pv tomato.
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Copyright © 2003 New Zealand Plant Protection Society (Inc.).