New Zealand Plant Protection 57 (2004): 130-137
Optimisation of PTA-ELISA detection and quantification of Botrytis cinerea infections in grapes
F.O. Obanor, K. Williamson, D.C. Mundy, P.N. Wood and M. Walter
ABSTRACT
Botrytis cinerea can be detected in juice pre- and postharvest using the monoclonal antibody BC-12.CA4 in plate trapped antigen-enzyme linked immunosorbent assays (PTA-ELISA). The amount of antigen predicted by PTA-ELISA was found to increase as grape juice samples were progressively diluted. This effect was also observed in B. cinerea contaminated boysenberry and blackcurrant juice samples. Detection of B. cinerea infections by PTA-ELISA was improved by incubation of grapes at 20ºC for 48 h and was similar to visual assessments of infected grapes. PTA-ELISA is faster (1-3 days) than visual B. cinerea assessments (10-14 days). PTA-ELISA measurements can be standardised, removing human bias in determining B. cinerea infection levels.
Keywords: latent infection, monoclonal antibody, BC-12CA4, immunoassay, juice.
Copyright © 2004 New Zealand Plant Protection Society (Inc.).
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 | Rapid method for the detection and quantification of Botrytis cinerea in plant tissues F.O. Obanor, M. Walter, N.W. Waipara and R. Cernusko (2002) New Zealand Plant Protection 55: 150-153 |
© 2007 New Zealand Plant Protection Society (Inc.)